Curcumin inhibits the formation of capillary-like tubes

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Curcumin inhibits the formation of capillary-like tubes

Postby patoco » Sat Feb 03, 2007 4:44 pm

Curcumin inhibits the formation of capillary-like tubes by rat lymphatic endothelial cells

Curcumin inhibits the formation of capillary-like tubes by rat
lymphatic endothelial cells.

Matsuo M, Sakurai H, Koizumi K, Saiki I.
Department of Anatomy, Faculty of Medicine, University of Toyama,
Toyama, Japan; Division of Pathogenic Biochemistry, Institute of
Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

The natural pigments curcumin and berberine have been shown to exhibit
a variety of pharmacologic effects including anti-inflammatory,
anti-cancer, and anti-metastatic properties. Here, we investigated the
anti-lymphangiogenic effect with an in vitro tube-forming model using
conditionally immortalized lymphatic endothelial TR-LE cells, a newly
established cell line originating from the thoracic duct of a
transgenic rat expressing the temperature-sensitive SV40 large
T-antigen. Curcumin, but not berberine, exhibited a dose-dependent
inhibition of the formation of capillary-like tubes by TR-LE cells
without affecting cell viability and adhesion to Matrigel. To address
the molecular mechanisms involved, we performed experiments with
specific inhibitors against putative targets of curcumin, including
IkappaB kinase (IKK), epidermal growth factor receptor (EGFR),
phosphatidylinositol-3 kinase (PI3K)/Akt, and matix metalloproteinases
(MMPs). While the IKK-2 inhibitor VI and EGFR tyrosine kinase
inhibitors gefitinib and PD153035 had no effect, both the PI3K
inhibitor LY294002 and the MMP inhibitor GM6001 shortened the tubes by
approximately 50%. Western blot analysis and gelatin zymography
revealed that curcumin, but not berberine, has an inhibitory effect on
the phosphorylation of Akt and enzymatic activity of MMP-2 in TR-LE
cells. These results suggest that curcumin exerts its inhibitory effect
on lymphangiogenesis partly through Akt and MMP-2.

PMID: 17197075 [PubMed - as supplied by publisher] ... etrieve&...


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