Expression of VEGF-C angiogenesis, lymphangiogenesis Cancer

[patoco]

Expression of VEGF-C and angiogenesis, and lymphangiogenesis in papillary thyroid carcinoma

August 2006

Liang QC, Wei QY, Fan SQ. Department of Pathlology, Second Xiangya Hospital, Central South University, Changsha, China.

OBJECTIVE: To investigate the relationship between the expression of vascular
endothelial growth factor C (VEGF-C) and angiogenesis and
lymphangiogenesis in papillary thyroid carcinoma (PTC).

METHODS:

Seventy-two PTC cases were divided into 3 groups according to the level
of invasion: papillary microcarcinoma group (PMC group), intrathyroid
carcinoma group (IPC group), and extrathyroid carcinoma group (EPC
group). They were again divided into 2 groups according to lymph node
metastasis: lymph node metastasis group and lymph node no-metastasis
group. The expressions of VEGF-C, CD105 and vascular endothelial growth factor receptor-3 (VEGFR-3) were detected by SP method of
immunohistochemical staining. The expression of VEGF-C was analyzed
quantitatively by image analysis system, and the PI of VEGF-C
(VEGF-C-PI), the number of MVD (microvessel density), and LVD
(lymphaticvessel density) were obtained.

RESULTS:

The VEGF-C-PI of lymph node metastasis group (23.15 +/- 3.75) was
higher than that of lymph node non-metastasis group (14.54 +/- 2.93) (P
<0.01). MVD was 35.25 +/- 2.06 in the PMC group, 41.75 +/- 5.46 in the
IPC group, and 52.58 +/- 4.16 in the EPC group, which showed the
elevatory tendency with the increase of invasion (P < 00.5). LVD was
6.00 +/- 0.81 in the PMC group, 13.80 +/- 1.81 in the IPC group, and
19.17 +/- 2.96 in the EPC group, which again showed the elevatory
tendency with the increase of invasion (P <0.05). The LVD of lymph node
metastasis group (19.56 +/- 2.45) was significantly higher than that of
lymph node non-metastasis group (12.48 +/- 2.84) (P < 0.05). VEGF-C was positively correlated with MVD and LVD (r = 0.743, 0.90, P <0.01).

CONCLUSION:

The expressions of VEGF-C and LVD are related to lymph node metastasis
of PTC. MVD and LVD are related to the invasion of PTC. VEGF-C may play an important role in the angiogenesis and lymphangiogenesis.

PMID: 16859137 [PubMed - in process]

http://www.ncbi.nlm.nih.gov/entrez/quer ... etrieve&...

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Related Abstract

Lymphangiogenesis and angiogenesis in bladder cancer: prognostic implications and regulation by vascular endothelial growth factors-A, -C, and -D.

Miyata Y, Kanda S, Ohba K, Nomata K, Hayashida Y, Eguchi J, Hayashi T,
Kanetake H.
Department of Urology, Nagasaki University School of Medicine,
Sakamoto, Nagasaki, Japan. int.doc.m...@m3.dion.ne.jp

PURPOSE:

Lymph vessel density (LVD) and microvessel density (MVD)
correlate with the malignant potential of tumors and patient survival.
Vascular endothelial growth factors (VEGF)-A, VEGF-C, and VEGF-D could
modulate LVD and MVD. We investigated the clinical and prognostic
significance of LVD and MVD on lymphangiogenic and angiogenic function
of VEGF-A, VEGF-C, and VEGF-D in human bladder cancer.

EXPERIMENTAL DESIGN:

We reviewed tissue samples from patients with
nonmetastatic bladder cancer who had undergone transurethral resections
(n = 126). The densities of D2-40-positive vessels (LVD) and
CD34-positive vessels (MVD) were measured by a computer-aided image
analysis system. Expression of VEGF-A, VEGF-C, and VEGF-D was examined by immunohistochemistry; survival analyses and their independent roles were investigated using multivariate analysis models.

RESULTS:

LVD was associated with tumor grade but not with pT stage. LVD
was associated with metastasis-free survival (log rank P = 0.039), but
was not an independent prognostic factor. Although MVD affected
survival, the combination of high LVD and high MVD in tumors was an
independent predictor of metastasis-free survival. Although VEGF-C
expression was positively associated with both LVD and MVD, VEGF-D was
associated only with LVD. VEGF-A expression was associated with MVD in
univariate analysis, however, it was not an independent factor.

CONCLUSIONS:

Lymphangiogenesis and angiogenesis influence
metastasis-free survival, and are regulated by VEGF-C and/or VEGF-D.
Our results suggest that LVD and MVD are useful tools for the selection
of postoperative management and treatment strategies in patients with
bladder cancer.

PMID: 16467091 [PubMed - indexed for MEDLINE]

http://clincancerres.aacrjournals.org/c ... t/12/3/800

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Vascular endothelial growth factor-C (VEGF-C) expression predicts lymph
node metastasis of transitional cell carcinoma of the bladder.

Suzuki K, Morita T, Tokue A.
Department of Urology, Jichi Medical School, Tochigi, Japan.
s.k...@jichi.ac.jp

PURPOSE: It has been found that expression of vascular endothelial growth factor-C (VEGF-C) in several carcinomas is significantly associated with angiogenesis, lymphangiogenesis and regional lymph node metastasis. However, VEGF-C expression in bladder transitional cell carcinoma (TCC) has not yet been reported. To elucidate the role of VEGF-C in bladder TCC, we examined VEGF-C expression in bladder TCC and pelvic lymph node metastasis specimens obtained from patients who
underwent radical cystectomy.

METHODS: Eighty-seven patients who underwent radical cystectomy for clinically organ-confined TCC of the bladder were enrolled in the present study. No neoadjuvant treatments, except transurethral resection of the tumor, were given to these patients. The VEGF-C expressions of 87 bladder tumors and 20 pelvic lymph node metastasis specimens were examined immunohistochemically and the association between VEGF-C expression and clinicopathological factors, including angiogenesis as evaluated by microvessel density (MVD), was also examined.

RESULTS: Vascular endothelial growth factor-C expression was found in the cytoplasm of tumor cells, but not in the normal transitional epithelium. Vascular endothelial growth factor-C expression was significantly associated with the pathological T stage (P = 0.0289), pelvic lymph node metastasis (P < 0.0001), lymphatic involvement (P = 0.0008), venous involvement (P = 0.0002) and high MVD (P = 0.0043).

The multivariate analysis demonstrated that VEGF-C expression and high MVD in bladder TCC were independent risk factors influencing the pelvic
lymph node metastasis. Moreover, the patients with VEGF-C-positive
tumors had significantly poorer prognoses than those with the
VEGF-C-negative tumors (P = 0.0087) in the univariate analysis. The
multivariate analysis based on Cox proportional hazard model showed
that the independent prognostic factors were patient age (P = 0.0132)
and pelvic lymph node metastasis (P = 0.0333).

CONCLUSION: The present study suggests that VEGF-C expression is an important predictive factor of pelvic lymph node metastasis in bladder cancer patients.

http://www.blackwell-synergy.com/doi/ab ... 2005.010...

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