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Lymphatic vessels, VEGFC/D in human skeletal muscles

PostPosted: Wed Sep 20, 2006 1:06 pm
by patoco
Localisation of lymphatic vessels and vascular endothelial growth factors-C and -D in human and mouse skeletal muscle with immunohistochemistry.

Kivela R, Havas E, Vihko V.
LIKES Research Center, Rautpohjankatu 8a, 40700, Jyvaskyla, Finland.

Aug 19, 2006

The present study was aimed to localise lymphatic vessels and their
growth factors in human and mouse skeletal muscle with
immunohistochemistry and specific antibodies (VEGFR-3, LYVE-1, VEGF-C
and VEGF-D). The largest lymphatic vessels were found in perimysial
connective tissue next to the arteries and veins, as has been shown
earlier with electron microscopy. As a new finding, we also found small
LYVE-1 positive vessels in the capillary bed between muscle fibres.
These vessels were located next to CD31 positive blood capillaries and
were of the same size, but fewer in number.

In addition, we described the localisation of the two main lymphangiogenic growth factor proteins, vascular endothelial growth factor-C and -D. Both proteins were expressed in skeletal muscle at mRNA and protein levels. VEGF-D was located under the sarcolemma in some of the muscle fibres, in the endothelia of larger blood vessels and in fibroblasts. VEGF-C protein was localised to the nerves and muscle spindles, to fibroblasts and
surrounding connective tissue, but was not found in muscle fibres or
endothelial cells.

Our results are the first to suggest the presence of
lymphatic capillaries throughout the skeletal muscle, and to present
the localisation of VEGF-C and -D in the muscles.

PMID: 16924525 [PubMed - as supplied by publisher]


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