Two-Color Lymphatic Mapping Ig-Conjugated Infrared Optics

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Two-Color Lymphatic Mapping Ig-Conjugated Infrared Optics

Postby patoco » Fri May 25, 2007 8:14 am

Two-Color Lymphatic Mapping Using Ig-Conjugated Near Infrared Optical Probes.

J Invest Dermatol. 2007 May 24

Hama Y, Koyama Y, Urano Y, Choyke PL, Kobayashi H.
1Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, USA.

To map the drainage patterns of the lymphatic system, multicolor optical probes must be developed, which can be interstitially administered. These agents must be of sufficient size to be retained by the lymphatic system and permit the conjugation or incorporation of fluorescent probes with varying emission wavelengths.

Quantum dots fulfill these criteria, but their potential toxicity limits their application to research settings. Here, we describe the synthesis of lymphatic optical probes based on Igs conjugated with Cy5.5 and Cy7 and demonstrate in animal models that these agents can map the lymphatic drainage patterns within axillary nodes draining both the breast and upper extremity, cervical nodes draining both the ear and upper extremity, and sentinel lymph nodes draining different anatomic locations. The ability to separately and simultaneously visualize the drainage patterns from two separate lymphatic vessels may have implications for the preoperative mapping of lymph nodes before lymph node resection.

The biocompatibility of fluorescently labeled Igs in comparison to other nanoparticulate fluorophores improves the chances of clinical translation.

This noninvasive and biocompatible multicolor method of optical lymphangiography may elucidate the complex human lymphatic system and reduce the risk of lymphedema after surgery for melanoma and other cancers.

Journal of Investigative Dermatology advance online publication, 24 May 2007; doi:10.1038/sj.jid.5700892.

PMID: 17522707 [PubMed - as supplied by publisher] ... med_docsum


Simultaneous two-color spectral fluorescence lymphangiography with near infrared quantum dots to map two lymphatic flows from the breast and the upper extremity.

Hama Y, Koyama Y, Urano Y, Choyke PL, Kobayashi H.
Molecular Imaging Program, Center for Cancer for Cancer Research, National Cancer Institute, NIH, Bldg. 10, Room 1B40, MSC 1088, Bethesda, MD, 20892-1088, USA,

Keywords: Lymphatic drainage - Imaging - Breast cancer - Lymph node - Lymphedema - Nanotechnology - Quantum dots - Spectral fluorescence imaging - Near infrared

Due to their small size and poor access, the lymphatic function has been difficult to study in vivo. Especially difficult is the mapping of lymphatic drainage from two basins into the same node.

Quantum dots can be used to perform multicolor images with high fluorescent intensity and are of a nano-size size suitable for lymphatic imaging via direct interstitial injection.

Here we show simultaneous two-color in vivo wavelength-resolved spectral fluorescence lymphangiography using two near infrared quantum dots with different emission spectra, which allow non-invasive and simultaneous visualization of two separate lymphatic flows draining the breast and the upper extremity and variations in the drainage patterns and the water sheds within the axillary node.

Two-color spectral fluorescence lymphangiography can provide insight into mechanisms of drainage from different lymphatic basins that may lead to sentinel lymph nodes detection of the breast cancer as well as prevention of complications such as lymphedema of the arm.


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