Monitoring of Microlymphatic Disturbances Lymphedema

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Monitoring of Microlymphatic Disturbances Lymphedema

Postby patoco » Fri May 25, 2007 1:45 am

Optical Monitoring of Microlymphatic Disturbances during Experimental Lymphedema.

Lymphat Res Biol. 2007 Mar

Philips Classic Laser Laboratories, University of Arkansas for
Medical Sciences (UAMS), Little Rock, Arkansas., Department of Optics
and Biomedical Physics, Research-Educational Institute of Optics and
Biophotonics, Saratov State University, Saratov, Russia.

Background: Rat mesentery has been widely used to study microvascular functions. The goal of this work is to extend this animal model to monitor blood and lymph microvessel function during lymphedema.

Methods and Results: Lymphedema is created by microsurgical removal of regional lymph nodes (lymphadenectomy) or ligation of the
collecting vein. Water volume in mesenteric tissue, microvessel
diameters, phasic contraction, valve function, lymph flow velocity,
and cell migration were analyzed during lymphedema development.

Dynamic observation of water amount after lymphadenectomy revealed
increasing edema from 30 min to 1 week; greatest degree of edema at
one week, and gradual decrease in edema from 1 to 11 weeks. These
effects were accompanied by acute constriction of lymph vessels and
slowing of lymph flow velocity, switching to dilation and appearance
of new blood capillaries at week 1, progressing to dilation and
degenerative changes of the microlymphatic wall at week 4, and,
finally, leading to lymphatic fibrosis and lymphangiogenesis at week

Acute venous insufficiency (30 min after vein ligation) led to
significant edema, decreasing blood flow velocity to stasis, and
output of erythrocytes from venules to interstitium, with further
movement to microlymphatics and regional lymph nodes.

Conclusions: Rat mesentery as an animal model in combination with an advanced optical imaging system is valuable in studying microlymphatic disturbances in mesentery during the development of
experimental lymphedema from latent period to chronic stages,
including monitoring of individual cell dislocation with high
resolution optical imaging.

PMID: 17508899 [PubMed - as supplied by publisher]
db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17508899&itool=icon abstr&query_hl=1&itool=pubmed_docsum


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